Graphene based gene transfection

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WebVisualizing The Localization Of Transfection Complexes During Graphene Nanoparticle-Based Transfection Journal of Materials Chemistry B … WebApr 12, 2024 · Polymeric gene carriers target the nucleus and express their therapeusis through transcription and translation . Gene carriers are either viral vectors (retro-viruses and adenoviruses) or non-viral vectors. Viral vectors submit their genetic materials to the host cells and are known to attain greater transfection . 18.3.1.2 Polymeric Micelles

Enhanced Cancer DNA Vaccine via Direct Transfection to Host …

WebJul 30, 2024 · Methods: Graphene oxide/APTES was modified by spermine (GOAS) and characterized by FT-IR, DLS, SEM and AFM techniques. Then pEGFP-p53 was loaded on GOAS, transfected into cells and evaluated by fluorescent microscopy and gene expression techniques. Results: FT-IR data approved the GOAS sheet formation. Ninety percent of … WebObjectives: This review describes the different dimensions of carbon-based nanocarriers including 0-D fullerene, 1-D Carbon Nanotubes (CNTs), and 2-D graphene and Graphene Oxide (GO) and their surface modification with different biocompatible and biodegradable molecules via covalent or non-covalent functionalization. phillies blogs

Functionalization of graphene for anticancer drug delivery IJN

WebApr 1, 2024 · Delivery of functional genes into stem cells shows great application prospect in DNA-based tissue engineering. However, comparing with epithelial cells and cancer cells, stem cells usually exhibit low gene transfection efficiency. To enhance the transfection efficiency, non-viral gene delivery in co … WebJan 27, 2011 · Our results suggest graphene to be a novel gene delivery nano-vector with low cytotoxicity and high transfection efficiency, promising for future applications in non … WebSep 15, 2012 · Efficient DNA delivery is essential for introducing new genes into living cells. However, effective virus-based systems carry risks and efficient synthetic systems that are non-toxic remain to be discovered. … phillies box score for today\u0027s game

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Graphene Oxide Incorporated PLGA Nanofibrous Scaffold for

WebGene therapy has been found to hold tremendous potential for the treatment of cancers beyond conventional methods. 30–32 Nonviral vector provides safer methods without the potential side effects of viral transfection, such as immunogenicity and carcinogenesis. 33 GO has been considered an ideal vector for gene delivery. 34,35 In this study ... WebTo evaluate the transfection efficiency of NGQDs, we delivered mRNA into HeLa cells with NGQDs. As shown in Figure 4 a, the NGQDs formed complexes with mRNA at a 20:1 … phillies bobblehead collectionWebMany researchers have tried to transfect genes using carbon-based materials such as graphene oxides (GOs) and graphene quantum dots (GQDs) [29,30,31,32]. ... Prior to in vitro gene transfection with NGQDs, we verified the cytotoxicity of the NGQDs via a CCK-8 assay kit. For the CCK-8 assay, various concentrations of NGQDs from 1 μg/mL to 1000 ... trying to find address of someone

"WebDec 7, 2024 · Graphene has been widely studied for biomedical use since its first appearance in 2004 [3], partly owing to its high Young's modulus, high fracture strength, high electrical conductivity, and excellent optical performance. Graphene also possesses a large specific surface area for adsorption of bioactive compounds and has high optical … " - Graphene based gene transfection

Graphene based gene transfection

Graphene materials as 2D non-viral gene transfer vector platforms …

WebOur results suggest graphene to be a novel gene delivery nano-vector with low cytotoxicity and high transfection efficiency, promising for future applications in non-viral based … WebApr 20, 2024 · Graphene-based nanomaterials have been used as scaffolds for cell culture and tissue engineering and have exhibited minimal harmful effect on the mammalian cells, very good adhesion properties, excellent gene transfection efficacy, high capability of the promotion of differentiation of stem cells into bone cells in a controlled manner for bone ...

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WebJan 21, 2016 · During PhD work, I extensively involved on the development of chitosan based novel non-viral vectors for gene therapy application. I developed polyamidoamine (PAMAM) dendrimer conjugated chitosan copolymer through various chemical methods and showed very high transfection efficiency. All my research works published in reputed … WebWe conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform …

WebJun 10, 2013 · Graphene oxide (GO) has been extensively explored in nanomedicine for its excellent physiochemical, electrical, and optical properties. ... 1 Jiangsu Key Laboratory for Carbon-Based, Functional Materials & Devices, ... remarkably enhanced plasmid DNA transfection efficiencies induced by the NIR laser are achieved using NGO-PEG-PEI as …

WebJun 1, 2014 · Abstract. Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also … WebJun 18, 2024 · Graphene-oxide-based nanocarriers have shown great potential in gene transfection and could be used in breast cancer treatments by overcoming the limitations and side effects of chemotherapy. This carbon-based material allows a facile surface modification to enhance its biological application and gene delivery efficiency.

WebMar 10, 2011 · Graphene is successfully used as a non-toxic nano-vehicle for efficient gene transfection and suggested to be a novel gene delivery nano-vector with low …

WebJan 6, 2024 · Graphene oxide for transfection. ThermoFisher Scientific describes transfection as the process of artificially introducing nucleic acids – DNA or RNA – into cells, utilising means other than viral infection. A paper published in Nanoscale 2011 titled ‘Graphene based gene transfection’ showed that graphene oxide (“GO”) bound with ... trying to find a house to rentWebAug 18, 2024 · Deoxyribonucleic acid (DNA) vaccines are a promising cancer immunotherapy approach. However, effective delivery of DNA to antigen-presenting cells (e.g., dendritic cells (DCs)) for the induction of an adaptive immune response is limited. Conventional DNA delivery via intramuscular, intradermal, and subcutaneous injection by … trying to find a church on vacationWebDec 4, 2024 · The objective of this study is to design a graphene-based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene … phillies box score from todayWebGraphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we … trying to find a family memberWebFeb 28, 2024 · Feng L Zhang S Liu Z Graphene based gene transfection Nanoscale 2011 33 1252 1257 21270989 , [Web of Science ®], [Google Scholar] Yang X Wang Y Huang X Multi-functionalized graphene oxide based anticancer drug-carrier with dual-targeting function and pH-sensitivity J Mater Chem 2011 2110 3448 3454 , [Web of Science ®], … phillies booksWebDec 1, 2024 · Graphene and its derivatives, as sheet-like carbonic nanomaterials, have been progressively utilized in various biomedical fields. Currently, applications of … phillies box score last night\\u0027s gameWebJan 10, 2014 · Successful gene therapy requires efficient and safe gene vectors that protect DNA from nuclease degradation as well as facilitate DNA uptake with high transfection efficiency [139], [140]. Graphene has been explored for applications in gene delivery, gene–drug co-delivery and protein delivery with good supportive literature available. phillies boxy pinstripe shirt