Jc 1 staining principle

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August undefined, 2024

WebAll staining procedures must be performed without direct exposure to intense light, as JC-1 is light sensitive. If staining is too intense, bring down the final JC-1 concentration may... WebThe cyanine dye [ 1] JC-1 has been used to determine mitochondrial transmembrane potential. [ 2] [ 3] [ 4] A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λ ex 520 nm) monomer at low membrane potential.

Analysis of the Mitochondrial Membrane Potential Using the

WebJC-1 is a fluorescent compound that accumulates in mitochondria according to their transmembrane potentials. But lox potential mitochondria are usually bigger, and higher … WebThe JC-1 staining protocol is very simple: - wash cells in dilution buffer or PBS - add JC solution - incubate for 30 min at 37ºC for suspension cells, or 10 min for adherent cells - wash cells with dilution buffer - treat cells as … george orwell what is fascism

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WebIt changes to monomeric form and stains cells in green fluorescence. Although JC-1 is widely used in many labs, its poor water solubility causes great inconvenience. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 is developed to be a superior alternative to JC-1 when high dye concentration is desired. WebJC-1 (CBIC2) is an ideal fluorescent probe widely used to detect mitochondrial membrane potential. JC-1 accumulates in mitochondria in a potential dependent manner and can be used to detect the membrane potential of cells, tissues or purified mitochondria. WebMay 9, 2024 · Mitochondrial function was evaluated by JC-1 staining. Double immunofluorescence labeling of LC3 and TOMM20 used to assess the degree of … christian book store adrian mi

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Mitochondria Staining Kit (CS0390) - Technical Bulletin

WebDec 26, 2015 · 5. Wash each staining jar with three (3) changes of 1% Calcium Chloride for a total of approximately ten (10) minutes. 6. Add 2% Cobalt Chloride to each jar for ten (10) minutes. 7. Wash with three (3) to five (5) changes of an approximately 1:20 solution of 0.1M Sodium Barbital (~ 5 ml 0. 1 M Sodium Barbital + deionized water ~ 100 ml). WebJan 1, 2024 · JC-1 can be used to assess the ΔΨMboth in intact isolated mitochondria and tissues. In fact, the JC-1 dye is a lipophilic, cationic dye (naturally exhibiting green fluorescence) which is able to enter into the mitochondria where it accumulates and (in a concentration-dependent manner) starts forming reversible complexes called J aggregates. george orwell who controls the past quoteWeb2. JC-1 Staining Solution (2X) Preparation Thaw JC-1 Reagent (Item No. 10009908) at room temperature. Mix well to ensure there are no particles or flakes in the solution. Make a 2X staining solution by diluting the stock vial 1:100 into complete culture medium (containing FBS), and keep in the dark at room temperature until use. christian books to help with depression

"WebJC-1 Staining Solution Preparation Thaw an aliquot of the JC-1 Reagent (Item No. 10009908) at room temperature. Prepare a staining solution by diluting the reagent 1:10 … " - Jc 1 staining principle

Jc 1 staining principle

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WebJC-1) to green fluorescence (JC-1 monomers). This kit enables a fast and simple staining of isolated mitochondria from animal tissues and cell lines. It includes valinomycin, a mitochondrial membrane-dissipating agent, for control experiments. Components The kit is sufficient for 50 staining assays of 2 ml using JC-1 Stain 25 g WebJC-1 Dye for Mitochondrial Membrane Potential Thermo Fisher Scientific - KR Popular TaqMan Real-Time PCR Assays Antibodies Oligos, Primers & Probes GeneArt Gene …

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WebJC-1 BH-3 profiling is best suited to cell lines or homogenous samples because it provides a bulk response and not a single cell measurement. It is one of the easiest methods to use, … WebMay 9, 2024 · In the normal state, JC-1 aggregates in the mitochondrial matrix to form polymers, producing red fluorescence signals; when mitochondrial membrane potentials decrease, JC-1 disaggregates to monomers which produce green fluorescence. Assays were conducted as follows.

WebMar 15, 2024 · At present, examination by CASA combined with cluster analysis of kinetic subgroups, JC-1 staining and COMET assay or staining with toluidine blue seem most appropriate; however, cell volumetry and other functional tests deserve better attention. ... Amann, R.P.; Pickett, B.W. Principles of cryopreservation and a review of cryopreservation … WebPrepare Staining Solution. Mix 25 µl of the 200× JC-1 Stock Solution in 4 ml of ultrapure water in an appropriate tube. Close the tube and mix the solution by inversion or vortex …

WebThe membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. JC-1 dye can be used as an indicator of mitochondrial membrane potential in a variety of cell types, including myocytes and neurons, as well as in intact … JC-1 is a novel cationic carbocyanine dye that accumulates in mitochondria. The … JC-1 dye exhibits potential-dependent accumulation in mitochondria, indicated … WebJC-1 is a cationic dye that accumulates in energized mitochondria. In apoptotic cells with low MMP, JC-1 remains in a monomeric form, which exhibits green fluorescence. While in …

WebJul 7, 2024 · JC-1, a cationic fluorescent dye when added to living cells, is known to be localized exclusively in mitochondria, particularly in good physiological conditions characterized by sufficient mitochondrial membrane potential (ΔΨ). The accumulation of JC-1 in these organelles leads to the formation J-ag …

WebFurthermore, another kind of dye, JC-1, yields green or red fluorescence depending on MMP and allows to distinguish cells with high or low mitochondria potential. Both measuring … george orwell war of the worlds broadcastWeb1.3 1 mM JC-1 Stock Solution: Allow the lyophilized vial JC-1 and the DMSO to warm to room temperature. Reconstitute the JC-1 by adding 766.8 µL DMSO to the 500 µg vial of JC-1. Any unused 1 mM JC-1 solution should be aliquoted and stored at -20 ºC. 1.4 Working JC-1 Solution: To prepare the Working JC-1 Solution, add the appropriate volume george orwell was born in what countryWebI understand JC-1 staining is used by many people to monitor mitochondrial membrane potential in apoptosis induction in a variety of cell systems. I see also different ways of interpreting the ... christian books to read for book clubWebAug 1, 2011 · 2011 Aug 1;2011(8):990-2. doi: 10.1101/pdb.prot5648. Author Brad Chazotte. PMID: 21807856 DOI: 10.1101/pdb.prot5648 Abstract INTRODUCTION Membrane-potential-dependent dyes such as Rhodamine 123, tetramethylrhodamine methyl ester (TMRM), and tetramethylrhodamine ethyl ester (TMRE) are useful as long as the mitochondrion … christian bookstore acworth gaWebJC-1 is a fluorescent compound that accumulates in mitochondria according to their transmembrane potentials. But lox potential mitochondria are usually bigger, and higher potential mitochodria... christian bookstore aiken scWebJan 1, 2024 · JC-1 can be used to assess the ΔΨ M both in intact isolated mitochondria and tissues. In fact, the JC-1 dye is a lipophilic, cationic dye (naturally exhibiting green … christian bookstore albany gaWebOn the basis of this phenomenon, several fluorescent probes, including rhodamine 123 and JC-1, have been developed to monitor the function of mitochondria in living cells (Smiley et al., 1991; Johnson et al., 1980, 1981). christian books to read as a family